• 姓       名:包建民
  • 职       称:教授
  • 学       位:博士
  • 所在机构:天津大学 药物科学与技术学院
  • 出生年月:
  • 籍       贯:安徽省巢湖市
  • 研究方向:生物化学;分析化学;蛋白质药物
个人简介 学术成果 发表论文

教育背景

1983年山东大学化学系理学学士

1986年山东大学化学系理学硕士

1993年美国普渡大学理学博士

工作经历

2002年中国教育部“奖励计划”天津大学特聘教授

2003年现任天津大学药学院教授,博士研究生导师

1993-2000年受聘于美国宝洁公司药厂作为科学家和资深科学家,从事药物分析的研究工作

2000-2003年任美国赛万事公司资深科学家,分析研发部经理

论文专著

已发表著作章节4篇,国内外杂志学术论文60余篇。近年在国内外作学术报告多次,并被国外数种学术刊物邀请通讯评委。代表性著作,如下:

(1)Bao, J., Silicone elastomers and their applications in medicine, the Collection of Graduate Student Papers, Shandong University, 1985,1: 122.

(2)Towns, J. K., Bao, J. and Regnier, F. E., Synthesis and evaluation of epoxy polymer phases for the analysis of proteins by capillary zone electrophoresis, J. Chromatogr. A, 1992, 599: 227-237.

(3)Bao, J. and Regnier, F. E., Ultramicro enzyme assays in a capillary electrophoretic system, J. Chromatogr. A, 1992, 608: 217-224.

(4)Bao, J., Homogeneous enzyme assays in capillary eletrophoresis, Ph.D. thesis, Purdue University, 1993, 234 pp. Univ. Microfilm Int., Order No. DA9513154, AN 1995: 738613.

(5)Miller, K., Leesong, I., Bao, J., Regnier, F. E. and Lytle, F. E., Electrophoretically mediated microanalysis of leucine aminopeptidase in complex matrices using time-resolved laser-induced fluorescence detection, Anal. Chem., 1993, 65: 3267-3270.

(6)Corey, A.*, Agnew, J., Bao, J., Bryson, P., Comer, P., Griffth, S., and Li, J. Effect of age and gender on azimilide pharmacokinetics after a single oral dose of azimilide dihydrochloride, J. Clin. Pharmacol., 1997, 37: 946-953.

(7)Bao, J.*, J. Fijima, and N. Danielson, Determination of minute enzymatic activities by means of capillary electrophoretic techniques, J. Chromatogr. B, 1997, 699: 481-497.

(8)Bao, J., Capillary electrophoretic immunoassays, J. Chromatogr. B, 1997, 699: 463-480.

(9)Bao, J.*, Parekh, N. and Shuja, A., Separation and quantitation of azimilide and its putative metabolites by capillary electrophoresis, J. Chromatogr. B., 1998, 720: 129-140.

(10)Bao, J., Separation of proteins by capillary electrophoresis using an epoxy based hydrophilic coating, J. Liq. Chromatogr. Relat. Technol., 2000, 23: 61-78.

(11)Danielson, N. D., Gallagher, P. A. and Bao, J. J.*, Chemical reagents and derivatization procedures in drug analysis, Encyclopedia of Anal. Chem., 2000, 7042-7076.

(12)Bao, J. J.* and Liu, Y. D., New multi-dimensional electrophoretic separation systems for genome and proteome studies,Frontiers of Biotechnology and Pharmaceuticals, 2000, 1: 1-13.

(13)Bao, J. J., Recent Development in the fabrication and applications of polymer microchips, Frontiers of Biotechnology and Pharmaceuticals, 2000,.

(14)Bao, J., Silica capillaries: epoxy coating, Encyclopedia of Chromatography, 2001, 4 (259): 774-776.

(15)Starkey, D. E., Han, A, Bao, J.J., Ahn, C. H., Wehmeyer, K.R.*, Prenger, M.C., Halsall, H.B., and Heineman, W.R., Fluorogenic assay for beta-glucuronidase using microchip-based capillary electrophoresis, J. Chromatogr. B., 2001, 762: 33-41.

(16)Dai, H. J., Parker, C. N., and Bao, J. J.*, Characterization and inhibition study of MurA enzyme by capillary electrophoresis, J. Chromatogr. B 2001,776: 123-132.

(17)Bao, J., Validation and application of a HPLC method for the quantitative analysis of azimilide and its metabolite in mouse blood, Frontiers of Biotechnology and Pharmaceuticals, 2002, 3.

(18)Dai, J., Tu, J., Anderson, L. N., Wehmeyer, K. R.*, Bao, J. J., and Liu, C. S., and Quay, B.,High-Throughput Separations: Using Multiplexed Capillary Electrophoresis with Laser-Induced Fluorescence Detection, a) LC*GC North America, 2002,20 (7), 606-617. b)Pharma Genomics, 2002, 26-57.

(19)Tu, J, Anderson, L. N, Dai, J., Peters, K., Carr, A., Loos, P., Buchanan, D., Bao, J. J., Liu, C. and Wehmeyer, K. R.*, Application of Multiplexed Capillary Electrophoresis with Laser Induced Fluorescence (MCE/LIF) Detection for the Rapid Measurement of Endogenous Extracellular Signal-Regulated Protein Kinase (ERK) Levels in Cell Extracts, J. Chromatogr. B., 2003, 789: 323-335.

(20)Starkey, D. E., Abdelaziez, Y., Ahn, C. H., Tu, J., Anderson, L., Wehmeyer, K.R.*, Izzo, N. J., Carr, A. N., Peters, K. G, Bao, J.J., Halsall, H.B., and Heineman, W.R., Determination of endogenous extracellular signal-regulated protein kinase by microchip capillary electrophoresis, Anal. Biochem., 2003, 316: 181-191.

(21)Bao, J.*, Villena, J., Wlasichuck, K. and Schmidt, D., High throughput determination of log d values by lc/ms method, in Chemical Analysis: A Series of Monographs on Analytical Chemistry and Its Applications, Analysis and purification methods in combinatorial chemistry, 2003, 435-455.

(22)Wu, J., Guo, Y. and Bao*, J., Applications of Electrospray in the Preparation of Nanomaterials, Bulletin of Materials, Special Volume for Nano and New Material, 2005, 19( 4): 8-11.

(23)Ding*, G., Tang, A. , Wang, J., Cong, R.., Bao, J., Direct Enantioseparation of Pantoprazole Sodium by High Performance Liquid Chromatography (HPLC) on Teicoplanin-bonded Chiral Stationary Phase, Chinese J. of Chromatogr., 2006, 24 (1):46-48.

(24)Ding*, G., Tang, A. , Wang, J., Cong, R.., Bao, J., Preparation of Teicoplanin-bonded Chiral Stationary Phase and its Application on Enantioseparation in Reversed Chromatography, Chinese Anal. Chem.,2006,34(3) 338-342.

(25)Y. Liu,G. Ding,J. Wu and J. Bao, New Flash Chromatography, Analytical Instruments, 2006.1, 12-15.

(26)X. Li and J. Bao*, Advancement of High-throughput Separation Technologies, Chemistry, 2006, 69 (2) : w013.

(27)Chen, Z.L., Liu, Y.X., Ding, G.S., Wu, J., and Bao*, J. J., Fast preparation and evaluation of capillaries covalently bonded with polyethyleneimide, Chinese J. Anal. Chem., 2006, 34(9), S214-S216.

(28)Ding, G., Da, Z., and Bao, J.*, Preparation and evaluation of mixed-mode silica monolithic column for capillary electrochromatography, Chinese Anal. Chem. 2006, 34(9), S68-S72.

(29)Fu, H-F, Guan, J., Qu, Z-S, Bao, J-M, Determination of dehydroandrographolide in compound Chuanxinlian tablets by hollow fiber solvent microextraction- HPLC, Chinese Journal of Pharmaceutical Analysis, 2007,27 (2) 222-224.

(30)Fu, H., Guan, J., Qu, Z. and Bao, J. J. *, Determination of Chlorhexidine Acetate in Chlorhexidine Acetate Suppositories by High Performance Liquid Chromatography Coupled with Hollow Fiber Membrane Solvent Microextraction, Chinese Chromatogr., 2006 24 (6): 566-569.

(31)Fu, H., Guan, J. and Bao, J. J. *, A Hollow Fiber Solvent Microextraction Approach to Measure Drug–protein Interactions, Anal. Sci., 2006, 22: 1565-69.

(32)Ding, G., Da, Z., Yuan, R., Bao*, J. J., Reversed Phase and Ion-exchange Mixed-mode Silica-Based Monolithic Column for Capillary Electrochromatography, Electrophoresis, 2006.9, 27,3363-3372.

(33)Guo, Y., Liu, D., Wang, H., Yuan, R. and Bao, J. J. *,Wide-bore Electrophoresis System with Single Capillary Inner-cooling and UV Detection, Chem. Lett., 2006, 35(12) :1386-1387.

(34)Yang, Y.and Bao, J. *, An electrical pumping approach to eliminate sample bias in capillary electrokinetic injection,Electrophoresis,2007,28(7), 1063-1671.

(35)Yuan, R., Ding, G., Guo, Y., Liu, D., and Bao, J. J. *, Wide bore monolithic column for electrochromatography, Electrophoresis, 2007, 28(11): 1674-1680.

(36)Liu, D., Bao*, J. J., Li, L., Lu, W., Liu, D. and Wehmeyer, K. R., Capillary Electrophoresis (CE) with Laser Induced Fluorescence (LIF) Detection for Enzyme Characterization and Inhibitor Screening,Anal. Sci., 2008, 24(3): 333-337.

(37)Liu D., Wang J., Guo Y., Yuan R., Wang H., and Bao J. J. *, Separation of aromatic acids by wide-bore electrophoresis with nano-particles prepared by electro-spray as pseudostationary phase, Electrophoresis,2008, 29(4): 863-870.

(38)Guo, Y., Liu, D., Wang, H., Yuan, R. and Bao, J. J. *, Zone electrophoresis on inner-cooling wide-bore electrophoresis system with UV detection, Anal. Sci., 2008, 24(8): 1025-1030.

(39)Li Q., Li X., Yang X., Bao J. and Shen X., Effects of antiallergic herbal agents on cystic fibrosis transmembrane conductance regulator in nasal mucosal epithelia of allergic rhinitis rabbits, Chin. Med. J., 2009, 122(24): 3020-3024.

(40)Song, W., Liu, Y., Wang, Q., and Bao, J. J. *, Preparation of novel stationary phase for solid phase extraction and its application for the analysis of benzoic acid in plum,Chinese J. Anal. Chem., 2009, 37(10):1507-1510.

(41)Li Q., Li X.L., Yang X., Bao J.J., Shen X.H., Effects of antiallergic herbal agents on cystic fibrosis transmembrane conductance regulator in nasal mucosal epithelia of allergic rhinitis rabbits, Chin Med J, 2009, 122 (24):3020-3024.

(42)Ding G., Jun L., and Bao J. J. *, Preparation and evaluation of amphiphilic silica-based monolithic column having surface-bound octanoyl-aminopropyl moieties for capillary electrochromatography, Electrophoresis, 2010, 31, 1983–1990.

(43)Li Y., Fang X., Zhao S., Zhai T., Sun X., and Bao J. J. *, Study on a Novel Two-dimensional HPLC and Wide-bore Electrophoresis System, Chem. Lett., 2010, 39: 983-985.

(44)Li Y., Dan Y., Fang X, and Bao J.J*, Preliminary studies of a novel multifunctional wide-bore electrophoresis system, Electrophoresis, 2010, 31: 3247-3255.

(45)Zhang B., Qi Y., Gao H., Bao J. J*, Application of solid phase extraction disk in the determination of acrylamide in tap water, Chinese Chromatogr., 2010. 28(12):1196-1199.

(46)Ding, G., Yuan, R., Da, Z. and Bao, J. J. *, Capillary electrochromatographic separation of enantiomers on a covalently bonded norvancomycin chiral monolithic column,Electrophoresis 2010, 31(12):1983-1990.

(47)Li Y., Liu D., and Bao J. J.*, Characterization of tyrosine kinase and screening enzyme inhibitor by capillary electrophoresis with laser-induced fluoresce detector, J. Chromatogr. B, 2011, 879, 107-112.

(48)Wang, J., Guo, Y., Yuan, R., Liu, D. and Bao, J. J. *, Efficiency enhancement in the capillary electrophoretic separation of aromatic acids with organo-silica nanoparticles as pseudo-stationary phase, J. Chromatogra. Sci., 2011, 49(1):51-56.

(49)Zhang B., Li Y., Gao H., Bian J., and Bao J.J. *, Rapid Determination of Protein Binding Constant by a Pressure-Mediated Affinity Capillary Electrophoresis Method,Electrophoresis, 2011, 32: 3589-3596.

(50)Li Q., Bao J.J., Li X.J., Zhang T., Shen X.H., Inhibiting effect of Astragalus polysaccharides on the functions of CD4+CD25highTreg cells in the tumor microenvironment of human hepatocellular carcinoma, Chin Med J, 2012, 125 (5):786-793.

专利成果

(1)Wen, X., Bao, J., Zhang, M. and Du, Z., Linear Polyurethane-polysiloxane Block Polymer for Medical Use, PI: CN 1032174 A 5 Apr. 1989, AI: CN 87-101388, 25 Sept. 1987. Faming Zhuanli Shenqing Gongkai Shuomingshu (Public instruction of patent), 13 pp. CA 113(22):198053n.

(2)Bao, J., Harmon, B. J., Patterson, D. H. and Regnier, F. E., Purdue Research Foundation, USA, Electrophoretically mediated chemical analysis, U. S. Patent 5,810,985, Sept. 22, 1998.

(3)Liu, Y. D. and Bao. J. J., Method and apparatus for interfacing multi-dimensional electrophoresis for automatic on-line analysis, US Patent, Serial No. 09/659420, September 11, 2000.

(4)Liu, Y. D. and Bao, J. J., High performance microbore electrophoresis, US Patent, Application No. 10/205579, July 24, 2002. PCT Appn.

(5)Liu, Y. D. and Bao. J. J., Method and apparatus for interfacing multi-dimensional electrophoresis for automatic on-line analysis, US Patent 6,676,819, January 13, 2004.

(6)Liu, Y. D. and Bao, J. J., High performance microbore electrophoresis, US Patent 6,974,528, Dec 13, 2005.

(7)Bao, J. J. and Fu, H., Hollow Membrane Tube Micro-Liquid-Liquid Extraction Device and its Applications, Chinese Patent,Application Date: 2 August 2005, Application No. 200510014701.1.

(8)包建民,付华峰,中空膜管式微萃取分析器及其应用方法,CN200510014701.1,2007-08-15

(9)包建民,张勃,李优鑫,测定受体配体间结合常数的压力驱动亲和毛细管电泳方法,发明专利,申请号:201110104249.3,发文日:2011.4.26

(10)包建民,周正昌,李优鑫,带有磁力搅拌功能可控温型微反应器的自动进样托盘,实用新型专利,专利号:ZL201220146084.6,授权日:2012.8.24

(11)包建民,李优鑫,陈志丹,一种提高非极性药物回收率的微透析装置,发明专利,申请号:201210299187.0,申请日:2012.8.21,发文日:2012.8.22

(12)包建民,李优鑫,贾菲菲,梁清刚,新型环糊精聚合物手性拆分剂的合成及应用,发明专利,申请号:201210430431.2,发文日:2012.11.2

(13)包建民,李优鑫,贾菲菲,梁清刚,王雨潇,一系列新型环糊精聚合物手性拆分剂的合成及应用,发明专利,申请号:201210430597.4,发文日:2012.11.2

(14)包建民,李优鑫,张勃,高赫男,一种在线转移和分析凝胶中蛋白质的装置和方法,发明专利,申请号:201210430431.2,发文日:2012.11.2

(15)包建民,李优鑫,周正昌,利用中空纤维膜测定口服药物渗透性系数的方法,发明专利,申请号:201310094976.5,发文日:2013.03.25

(16)包建民,续竟生,马志爽,张元津,李优鑫,一种样品制备仪,发明专利,申请号:201310050581.5,发文日:2013.2.16

(17)包建民,续竟生,马志爽,张元津,李优鑫,一种样品制备仪,实用新型专利,申请号:201320072541.6,发文日:2013.2.16

(18)包建民,周正昌,李优鑫,带有磁力搅拌功能可控温型微反应器的自动进样托盘,申请类型:发明专利,申请号:201210101275.5,授权日:2013.7.1