1998.9 - 2002.6 江南大学生物工程学院，工学学士
2002.9 - 2007.10 中科院微生物研究所，理学博士
2007.10 - 2009. 2 中南大学冶金科学与工程学院，讲师
2009. 2 - 2009. 7 湖南大学化学化工学院，讲师
2009. 7 - 2015. 9 湖南大学化学化工学院，副教授
2013.10 - 2014.10 美国加州大学圣地亚哥分校，访问学者
2015.10 - 至今 湖南大学化学化工学院，教授
1. 主持国家自然科学基金面上项目，基于重组近红外荧光蛋白的生物传感与活体成像研究，2017-2020;
2. 主持国家自然科学基金面上项目，基于超电荷荧光蛋白的核酸传感新方法，2015-2018;
3. 主持国家自然科学基金青年项目，基于双功能全细胞传感器的纳米材料生物毒性高通量筛查新方法，2009-2012;
4. 主持湖南省自然科学基金***项目，基于功能荧光蛋白的生化分析与生物传感研究，2017-2019;
5. 主持湖南省自然科学基金重点项目，新型污染物生物毒性高通量筛查的传感新方法，2010-2012;
6. 主持湖南大学青年教师成长计划，2013-2015;
7. 参与国家自然科学基金重大项目，核酸适配体的分析化学基础研究，2012-2016;
8. 参与国家重点基础研究发展计划(973项目)，超痕量POPs的快速筛查与生物分析方法，2009-2013。
1. K.L. Zhao, Y Tang, Z. Wang, J. Zhang, C.Y. Lei, H.H. Wang, H. Li, Yan Huang*, Z. Nie* , S.Z. Yao. Surface charge tuneable fluorescent protein-based logic gates for smart delivery of nucleic acids. Chem. Commun., 2017, 53, 11326.
2. M.Z. Yu, K.L. Zhao, X.H. Zhu, S.Y. Tang, Z. Nie, Yan Huang*, P. Zhao*, S.Z. Yao. Development of near-infrared ratiometric fluorescent probe based on cationic conjugated polymer and CdTe/CdS QDs for label-free determination of glucose in human body fluids. Bios. Bioelectron., 2017, 95, 41–47.
3. B.B Xiang, K.Y. He, R. Zhu, Z.L. Liu, S. Zeng, Yan Huang*, Z. Nie*, S.Z. Yao. Self-Assembled DNA Hydrogel Based on Enzymatically Polymerized DNA for Protein Encapsulation and Enzyme/DNAzyme Hybrid Cascade Reaction. ACS applied materials & interfaces, 2016, 8, 22801-22807.
4. C.Y. Lei, Z. Wang, Z. Nie, H.H. Deng, H.P. Hu, Yan Huang*, S.Z. Yao. Resurfaced Fluorescent Protein as a Sensing Platform for Label-Free Detection of Copper(II) Ion and Acetylcholinesterase Activity. Anal. Chem., 2015, 87, 1974−1980.
5. L.H. Lin, S.Q. Liu, Z. Nie, Y.Z. Chen, C.Y. Lei, Z. Wang, C. Yin, H.P. Hu, Yan Huang*, S.Z. Yao. Automatic and Integrated Micro-Enzyme Assay (AIμEA) Platform for Highly Sensitive Thrombin Analysis via an Engineered Fluorescence Protein-Functionalized Monolithic Capillary Column. Anal. Chem., 2015, 87, 4552−4559.
6. C. Yin, M. Wang, C.Y. Lei, Z. Wang, .Li, Y. Li, W. Li, Yan Huang*, Z. Nie*, S.Z. Yao. Phosphorylation-mediated Assembly of Semisynthetic Fluorescent Protein for Label-free Detection of Protein Kinase Activity. Anal. Chem., 2015, 87, 6311−6318.
7. Z. Wang, Y. Li, L.J. Li, D.Q. Li, Yan Huang*, Z. Nie*, S.Z. Yao. DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity. Chem. Commun., 2015, 51, 13373—13376.
8. L.J. Li, H. Lin, C.Y. Lei, Z. Nie, Yan Huang*, S.Z. Yao. Label-free fluorescence assay for thrombin based on unmodified quantum dots. Bios. Bioelectron., 2014, 54, 42–47.
9. Q.P. Shen, L.F. Zhou, Y.J. Yuan, Yan Huang*, B.B. Xiang, C.Y. Chen, Z. Nie*, S.Z. Yao. Intra-molecular G-quadruplex structure generated by DNA-templated click chemistry: “Turn-on” fluorescent probe for copper ions. Bios. Bioelectron., 2014, 55, 187–194.
10. F.F. Li, C.Y. Lei, Q.P. Shen, L.J. Li, M. Wang, M.L. Guo, Yan Huang*, Z. Nie*, S.Z. Yao. Analysis of Copper Nanoparticles Toxicity Based on a Stress-responsive Bacterial Biosensor Array. Nanoscale, 2013, 5(2), 653-662.
11. H. Lin, L.J. Li, C.Y. Lei, X.H. Xu, Z. Nie, M.L. Guo, Yan Huang*, S.Z. Yao. Immune-independent and label-free fluorescent assay for Cystatin C detection based on protein-stabilized Au nanoclusters. Bios. Bioelectron., 2013, 41, 256-261.
12. Yan Huang, K.X. Zhao, X.H. Shen, M. T. Chaudhry, C.Y. Jiang, and S.J. Liu*. Genetic Characterization of the Resorcinol Catabolic Pathway in Corynebacterium glutamicum. Applied and Environmental Microbiology. 2006. 72, 7238-7245.
1. 2017年入选湖南省自然科学基金***
2. 2015年入选湖南省普通高校青年骨干教师培养计划
